Multiday maintenance of extracorporeal lungs using cross-circulation with conscious swine.

ABSTRACT:

Objectives

Lung remains the least-utilized solid organ for transplantation. Efforts to recover donor lungs with reversible injuries using ex vivo perfusion systems are limited to <24 hours of support. Here, we demonstrate the feasibility of extending normothermic extracorporeal lung support to 4 days using cross-circulation with conscious swine.

Methods

A swine behavioral training program and custom enclosure were developed to enable multiday cross-circulation between extracorporeal lungs and recipient swine. Lungs were ventilated and perfused in a normothermic chamber for 4 days. Longitudinal analyses of extracorporeal lungs (ie, functional assessments, multiscale imaging, cytokine quantification, and cellular assays) and recipient swine (eg, vital signs and blood and tissu

SUBMITTER: Hozain AE 

PROVIDER: S-EPMC7094131 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature