Gut microbiota composition and frailty in elderly patients with Chronic Kidney Disease.

ABSTRACT:

Background

Frailty is common in older patients affected by chronic kidney disease (CKD). Since gut microbiota (gMB) may contribute to frailty, we explored possible associations between gMB and frailty in CKD.

Methods

We studied 64 CKD patients (stage 3b-4), categorized as frail (F, 38) and not frail (NF, 26) according to Fried criteria, and 15 controls (C), all older than 65 years. In CKD we assessed serum C-reactive protein, blood neutrophil/lymphocyte ratio, Malnutrition-inflammation Score (MIS); gMB was studied by denaturing gel gradient electrophoresis (DGGE), high-throughput sequencing (16S r-RNA gene), and quantitative real-time PCR (RT-PCR).

Results

No differences in alpha diversity between CKD and C and between F and NF patients emerged, but high-throughput se

SUBMITTER: Margiotta E 

PROVIDER: S-EPMC7112193 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature