Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle.

ABSTRACT: To identify host factors involved in Salmonella replication, SILAC-based quantitative proteomics was used to investigate the interactions of Salmonella typhimurium with the secretory pathway in human epithelial cells. Protein profiles of Golgi-enriched fractions isolated from S. typhimurium-infected cells were compared with those of mock-infected cells, revealing significant depletion or enrichment of 105 proteins. Proteins annotated to play a role in membrane traffic were overrepresented among the depleted proteins whereas proteins annotated to the cytoskeleton showed a diverse behavior with some proteins being enriched, others being depleted from the Golgi fraction upon Salmonella infection. To study the functional relevance of identified proteins in the Salmonella infection cycle, small

SUBMITTER: Vogels MW 

PROVIDER: S-EPMC7167899 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature