A Simple, Cost-Effective, and Robust Method for rRNA Depletion in RNA-Sequencing Studies.

ABSTRACT: The profiling of gene expression by RNA sequencing (RNA-seq) has enabled powerful studies of global transcriptional patterns in all organisms, including bacteria. Because the vast majority of RNA in bacteria is rRNA, it is standard practice to deplete the rRNA from a total RNA sample such that the reads in an RNA-seq experiment derive predominantly from mRNA. One of the most commonly used commercial kits for rRNA depletion, the Ribo-Zero kit from Illumina, was recently discontinued abruptly and for an extended period of time. Here, we report the development of a simple, cost-effective, and robust method for depleting rRNA that can be easily implemented by any lab or facility. We first developed an algorithm for designing biotinylated oligonucleotides that will hybridize tightly and specifi

SUBMITTER: Culviner PH 

PROVIDER: S-EPMC7175087 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature