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Single-cell chromatin immunocleavage sequencing (scChIC-seq) to profile histone modification.

ABSTRACT: Our method for analyzing histone modifications, scChIC-seq (single-cell chromatin immunocleavage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice by tethering to a specific antibody. Cleaved target sites are then selectively PCR amplified. We show that scChIC-seq reliably detects H3K4me3 and H3K27me3 target sites in single human white blood cells. The resulting data are used for clustering of blood cell types.

SUBMITTER: Ku WL 

PROVIDER: S-EPMC7187538 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Single-cell chromatin immunocleavage sequencing (scChIC-seq) to profile histone modification.

Ku Wai Lim WL   Nakamura Kosuke K   Gao Weiwu W   Cui Kairong K   Hu Gangqing G   Tang Qingsong Q   Ni Bing B   Zhao Keji K  

Nature methods 20190328 4

Our method for analyzing histone modifications, scChIC-seq (single-cell chromatin immunocleavage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice by tethering to a specific antibody. Cleaved target sites are then selectively PCR amplified. We show that scChIC-seq reliably detects H3K4me3 and H3K27me3 target sites in single human white blood cells. The resulting data are used for clustering of blood cell types. ...[more]

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