NAD⁺ biosynthesis in bacteria is controlled by global carbon/nitrogen levels via PII signaling.

ABSTRACT: NAD⁺ is a central metabolite participating in core metabolic redox reactions. The prokaryotic NAD synthetase enzyme NadE catalyzes the last step of NAD⁺ biosynthesis, converting nicotinic acid adenine dinucleotide (NaAD) to NAD⁺ Some members of the NadE family use l-glutamine as a nitrogen donor and are named NadE^Gln Previous gene neighborhood analysis has indicated that the bacterial nadE gene is frequently clustered with the gene encoding the regulatory signal transduction protein PII, suggesting a functional relationship between these proteins in response to the nutritional status and the carbon/nitrogen ratio of the bacterial cell. Here, using affinity chromatography, bioinformatics analyses, NAD synthetase activity, and biolayer inter

SUBMITTER: Santos ARS 

PROVIDER: S-EPMC7196632 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature