A comparison of high-throughput plasma NMR protocols for comparative untargeted metabolomics.

ABSTRACT:

Introduction

When analyzing the human plasma metabolome with Nuclear Magnetic Resonance (NMR) spectroscopy, the Carr-Purcell-Meiboom-Gill (CPMG) experiment is commonly employed for large studies. However, this process can lead to compromised statistical analyses due to residual macromolecule signals. In addition, the utilization of Trimethylsilylpropanoic acid (TSP) as an internal standard often leads to quantification issues, and binning, as a spectral summarization step, can result in features not clearly assignable to metabolites.

Objectives

Our aim was to establish a new complete protocol for large plasma cohorts collected with the purpose of describing the comparative metabolic profile of groups of samples.

Methods

We compared the conventional CPMG approach to a

SUBMITTER: Bliziotis NG 

PROVIDER: S-EPMC7196944 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature