Transcriptional profiling to identify the key genes and pathways of pterygium.

ABSTRACT:

Purpose

Pterygium results from a variety of biological pathways that are involved in the formation of ocular surface diseases. However, the exact pathogenesis of pterygium is still unclear. Our study focused on gene expression profiles to better understand the potential mechanisms of pterygium.

Methods

RNA sequencing experiments were performed on clinical pterygium tissues and normal conjunctival tissues. To identify the hub genes for the development of pterygium, we further conducted weighted gene co-expression network analysis (WGCNA). qRT-PCR was utilized to validate the dysregulation of the most significant differentially expressed genes (DEGs) and key hub genes in the independent subjects.

Results

A total of 339 DEGs (P-adjusted < 0.05 and log2 fold change [log2F

SUBMITTER: Chen Y 

PROVIDER: S-EPMC7204871 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature