Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus.

ABSTRACT:

Objective

We assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1).

Research design and methods

We randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm², energy density: 0.2 J/cm²) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 rece

SUBMITTER: Ebrahimpour-Malekshah R 

PROVIDER: S-EPMC7206914 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature