Isolation and structural identification of a new T1-conotoxin with unique disulfide connectivities derived from Conus bandanus.

ABSTRACT:

Background

Conopeptides are neuropharmacological peptides derived from the venomous salivary glands of cone snails. Among 29 superfamilies based on conserved signal sequences, T-superfamily conotoxins, which belong to the smallest group, include four different frameworks that contain four cysteines denominated I, V, X and XVI. In this work, the primary structure and the cysteine connectivity of novel conotoxin of Conus bandanus were determined by tandem mass spectrometry using collision-induced dissociation.

Methods

The venom glands of C. bandanus snails were dissected, pooled, and extracted with 0.1% trifluoroacetic acid in three steps and lyophilized. The venom was fractionated and purified in an HPLC system with an analytical reversed-phase C₁₈ co

SUBMITTER: Bao N 

PROVIDER: S-EPMC7216822 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature