Ontology highlight
ABSTRACT:
SUBMITTER: Tian S
PROVIDER: S-EPMC7228847 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Tian Songhai S Liu Yang Y Wu Hao H Liu Hao H Zeng Ji J Choi Mei Yuk MY Chen Hong H Gerhard Ralf R Dong Min M
Cell host & microbe 20200416 5
The exotoxin TcsL is a major virulence factor in Paeniclostridium (Clostridium) sordellii and responsible for the high lethality rate associated with P. sordellii infection. Here, we present a genome-wide CRISPR-Cas9-mediated screen using a human lung carcinoma cell line and identify semaphorin (SEMA) 6A and 6B as receptors for TcsL. Disrupting SEMA6A/6B expression in several distinct human cell lines and primary human endothelial cells results in reduced TcsL sensitivity, while SEMA6A/6B over-e ...[more]