Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry.

ABSTRACT:

Background

Reliable high-throughput microbial pathogen identification in human urine samples is crucial for patients with cystitis symptoms. Currently employed methods are time-consuming and could lead to unnecessary or inadequate antibiotic treatment. Purpose of this study was to assess the potential of mass spectrometry for uropathogen identification from a native urine sample.

Methods

In total, 16 urine samples having more than 10⁵ CFU/mL were collected from clinical outpatients. These samples were analysed using standard urine culture methods, followed by 16S rRNA gene sequencing serving as control and here described culture-independent MALDI-TOF/TOF MS method being tested.

Results

Here we present advantages and disadvantages of bottom-up proteomics, us

SUBMITTER: Oros D 

PROVIDER: S-EPMC7310424 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature