Single-cell sequencing of genomic DNA resolves sub-clonal heterogeneity in a melanoma cell line.

ABSTRACT: We performed shallow single-cell sequencing of genomic DNA across 1475 cells from a cell-line, COLO829, to resolve overall complexity and clonality. This melanoma tumor-line has been previously characterized by multiple technologies and is a benchmark for evaluating somatic alterations. In some of these studies, COLO829 has shown conflicting and/or indeterminate copy number and, thus, single-cell sequencing provides a tool for gaining insight. Following shallow single-cell sequencing, we first identified at least four major sub-clones by discriminant analysis of principal components of single-cell copy number data. Based on clustering, break-point and loss of heterozygosity analysis of aggregated data from sub-clones, we identified distinct hallmark events that were validated within bulk s

SUBMITTER: Velazquez-Villarreal EI 

PROVIDER: S-EPMC7316972 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature