Pleiotropic effects of vitamin D₃ on CD4⁺ T lymphocytes mediated by human periodontal ligament cells and inflammatory environment.

ABSTRACT:

Aims

Both, vitamin D₃ and human periodontal ligament cells (hPDLCs) possess immunosuppressive properties, but their combined effect on immune cells has never been investigated. Here, we analysed the impact of vitamin D₃ on the immunosuppressive properties of hPDLCs towards CD4⁺ T lymphocytes.

Material and methods

Allogenic CD4⁺ T lymphocytes were activated by phytohemagglutinin either in monoculture or co-culture with hPDLCs, in the presence or absence of IFN-γ and 1,25(OH)₂ D₃ . After 5 days, CD4⁺ T-lymphocyte proliferation, CD4⁺ CD25⁺ FoxP3⁺ regulatory T lymphocytes (T_regs ) proportion and IL-10, TGF-β1 and IL-17A production were analysed.

Results

SUBMITTER: Behm C 

PROVIDER: S-EPMC7318673 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature