Prognostic Value of Circulating Microvesicle Subpopulations in Ischemic Stroke and TIA.

ABSTRACT: Platelet microvesicles (PMV) have previously been found elevated in acute ischemic stroke (IS) and could be biomarkers for risk of recurrence. PMV surface antigens such as P-selectin and phosphatidylserine (PS) reflect platelet activation and procoagulance. Tissue factor-positive microvesicles (TF⁺MV) are considered procoagulant, in particular if co-expressing PS. We enumerated MV subpopulations with these surface antigens in a cohort of 211 patients with primarily non-cardioembolic IS or transient ischemic attack (TIA) and investigated their association with long-term outcome. MV concentrations were determined by flow cytometry in the acute and convalescent phase. Primary outcome was a composite of fatal and non-fatal recurrent IS or myocardial infarction. Secondary outcomes were recurrent IS and all-cause mortality. Outcome events were obtained from Swedish registers during a follow-up of 1100 patient years. Concentrations of PS-positive and PS-negative MV populations were elevated in patients compared with healthy controls in both the acute and convalescent phase. PS⁺TF⁺PMV displayed pronounced elevations, median fold change 77 in the acute phase (p < 0.0001) but were not associated with outcome, neither were PS⁺P-selectin⁺PMV. The only subpopulation positively associated with primary outcome was PS^-TF⁺PMV, with adjusted hazard ratio of 1.86 (1.04-3.31, p = 0.036) by Cox regression. Unexpectedly, several MV subpopulations tended to be associated with reduced risk of poor long-term outcome. Our results suggest that PS⁺TF⁺PMV may be a promising marker for cerebral ischemia, and that the in vivo generation of PS^-MV after IS/TIA warrants further study. Future MV studies should ideally enumerate PS⁺ and PS^-MV subpopulations separately.

SUBMITTER: Lundstrom A 

PROVIDER: S-EPMC7340656 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature