A novel humanized mouse model to study the function of human cutaneous memory T cells in vivo in human skin.

ABSTRACT: Human skin contains a population of memory T cells that supports tissue homeostasis and provides protective immunity. The study of human memory T cells is often restricted to in vitro studies and to human PBMC serving as primary cell source. Because the tissue environment impacts the phenotype and function of memory T cells, it is crucial to study these cells within their tissue. Here we utilized immunodeficient NOD-scid IL2rγ^null (NSG) mice that carried in vivo-generated engineered human skin (ES). ES was generated from human keratinocytes and fibroblasts and was initially devoid of skin-resident immune cells. Upon adoptive transfer of human PBMC, this reductionist system allowed us to study human T cell recruitment from a circulating pool of T cells into non-inflamed human ski

SUBMITTER: Klicznik MM 

PROVIDER: S-EPMC7341892 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature