Autologous Exosome Transfer: A New Personalised Treatment Concept to Prevent Colitis in a Murine Model.

ABSTRACT:

Background and aims

Epigenetic information delivered by intestinal exosomes can be useful for diagnosing intestinal diseases, such as ulcerative colitis, but the therapeutic effects of intestinal exosomes have not been fully exploited. We herein developed an autologous exosome therapy that could treat intestinal disease without any risk of inducing a systemic immunological reaction.

Methods

Intestinal exosomes were isolated and purified from faeces by our newly developed multi-step sucrose gradient ultracentrifugation method. Lipopolysaccharide [LPS]-activated macrophages were employed to test the in vitro anti-inflammatory ability of intestinal exosomes. To evaluate the in vivo anti-inflammatory activity of our system, we gavaged dextran sulphate sodium [DSS]-induced coliti

SUBMITTER: Yang C 

PROVIDER: S-EPMC7346889 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature