Comparison of Capillary and Schirmer Strip Tear Fluid Sampling Methods Using SWATH-MS Proteomics Approach.

ABSTRACT:

Purpose

The purpose of this study was to examine the protein profile differences between capillary and Schirmer strip tear fluid samples.

Methods

Both capillary and Schirmer strip tear samples were collected from 31 healthy participants at the same visit, and the samples were analyzed with nanoflow liquid chromatography coupled with time-of-flight mass spectrometer (NanoLC-MSTOF), implementing a sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). Sample type-specific and combined spectral libraries were used to evaluate the differences between the sample types in protein expression levels and biological functions.

Results

In proportion, more extracellular proteins connected to immune response were quantified from the cap

SUBMITTER: Nattinen J 

PROVIDER: S-EPMC7351636 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature