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Development and validation of an ambulatory piglet model for short bowel syndrome with ileo-colonic anastomosis.

ABSTRACT:

Impact statement

Short bowel syndrome is associated with significant comorbidities and mortality. This study is important as unlike current systems, it provides a validated piglet model which mirrors anatomical, histological, and serological characteristics observed in human SBS. This model can be used to advance knowledge into mechanistic pathways and therapeutic modalities to improve outcomes for SBS patients. This study is novel in that in addition to significant reduction in the remnant bowel and noted liver disease, we also developed a method to emulate ileocecal valve resection and described gut adaptive responses which has important clinical implications in humans.

SUBMITTER: Manithody C

PROVIDER: S-EPMC7357144 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Development and validation of an ambulatory piglet model for short bowel syndrome with ileo-colonic anastomosis.

Manithody Chandrashekhara C Denton Christine C Price Amber A Blomenkamp Keith K Patel Yogi Y Welu Adam A Glbert Ester E Madnawat Himani H Jain Sonali S Villalona Gustavo A GA Jain Ajay K AK

Experimental biology and medicine (Maywood, N.J.) 20200407 12

<h4>Impact statement</h4>Short bowel syndrome is associated with significant comorbidities and mortality. This study is important as unlike current systems, it provides a validated piglet model which mirrors anatomical, histological, and serological characteristics observed in human SBS. This model can be used to advance knowledge into mechanistic pathways and therapeutic modalities to improve outcomes for SBS patients. This study is novel in that in addition to significant reduction in the remn ...[more]

PMID: 32264693

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Gut microbial diversity is reduced and is associated with colonic inflammation in a piglet model of short bowel syndrome.

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| S-EPMC3669166 | biostudies-literature

Unravelling the metabolic impact of SBS-associated microbial dysbiosis: Insights from the piglet short bowel syndrome model.

Project description:Liver disease is a major source of morbidity and mortality in children with short bowel syndrome (SBS). SBS-associated microbial dysbiosis has recently been implicated in the development of SBS-associated liver disease (SBS-ALD), however the pathological implications of this association have not been explored. In this study high-throughput sequencing of colonic content from the well-validated piglet SBS-ALD model was examined to determine alterations in microbial communities, and concurrent metabolic alterations identified in urine samples via targeted mass spectrometry approaches (GC-MS, LC-MS, FIA-MS) further uncovered impacts of microbial disturbance on metabolic outcomes in SBS-ALD. Multi-variate analyses were performed to elucidate contributing SBS-ALD microbe and metabolite panels and to identify microbe-metabolite interactions. A unique SBS-ALD microbe panel was clearest at the genus level, with discriminating bacteria predominantly from the Firmicutes and Bacteroidetes phyla. The SBS-ALD metabolome included important alterations in the microbial metabolism of amino acids and the mitochondrial metabolism of branched chain amino acids. Correlation analysis defined microbe-metabolite clustering patterns unique to SBS-ALD and identified a metabolite panel that correlates with dysbiosis of the gut microbiome in SBS.

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Project description:Short bowel syndrome mouse microbiome

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Enhanced Ghrelin Levels and Hypothalamic Orexigenic AgRP and NPY Neuropeptide Expression in Models of Jejuno-Colonic Short Bowel Syndrome.

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Anorectal Function After Ileo-Rectal Anastomosis Is Better than Pelvic Pouch in Selected Ulcerative Colitis Patients.

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| S-EPMC6943403 | biostudies-literature

Pilot trial: pregabalin on colonic sensorimotor functions in irritable bowel syndrome.

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| S-EPMC3946640 | biostudies-literature

Satb2 deletion in colon approach to treat short bowel syndrome

Project description:Loss of the transcription factor Satb2 converts mouse colonic epithelium into an ileal type. Here, we report that Satb2 gene deletion alleviates SBS by replacing diminished absorptive function and by inducing lymphovascular channels in the proximal colon. Enhanced nutrient absorption following Satb2 loss increased body weight and survival of mice with SBS. Deletion of SATB2 by adeno-associated viral delivery of CRISPR Split Cas9 to human colon organoids, followed by xenotransplantation into mice, resulted in ileal morphology and expression of ileal marker genes. This approach offers a feasible strategy for future treatment of SBS.

2025-03-19 | GSE279770 | GEO

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