MALDI-MSI spatially maps N-glycan alterations to histologically distinct pulmonary pathologies following irradiation.

ABSTRACT: Radiation-induced lung injury is a highly complex combination of pathological alterations that develop over time and severity of disease development is dose-dependent. Following exposures to lethal doses of irradiation, morbidity and mortality can occur due to a combination of edema, pneumonitis and fibrosis. Protein glycosylation has essential roles in a plethora of biological and immunological processes. Alterations in glycosylation profiles have been detected in diseases ranging from infection, inflammation and cancer. We utilized mass spectrometry imaging to spatially map N-glycans to distinct pathological alterations during the clinically latent period and at 180 days post-exposure to irradiation. Results identified alterations in a number of high mannose, hybrid and complex N-glycans

SUBMITTER: Carter CL 

PROVIDER: S-EPMC7360629 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature