Evaluation of profibrotic gene transcription in renal tissues from cats with naturally occurring chronic kidney disease.

ABSTRACT:

Background

Increased gene transcription of hypoxia-induced mediators of fibrosis in renal tissue has been identified in experimentally induced, ischemic chronic kidney disease (CKD).

Objective

To characterize hypoxia-induced profibrotic pathways in naturally occurring CKD in cats.

Animals

Twelve client-owned cats with CKD and 8 healthy control cats.

Methods

In this prospective, cross-sectional study, bilateral renal tissue samples were assessed histologically for inflammation, tubular atrophy, and fibrosis, and by reverse transcription-quantitative PCR for characterization of transcript levels of hypoxia-inducible factor-1α (HIF1A), matrix metalloproteinases-2 (MMP2), -7 (MMP7), and -9 (MMP9), tissue inhibitor of metalloproteinase-1 (TIMP1), transforming growth

SUBMITTER: Lourenco BN 

PROVIDER: S-EPMC7379026 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature