High-throughput discovery of phage receptors using transposon insertion sequencing of bacteria.

ABSTRACT: As the most abundant microbes on Earth, novel bacteriophages (phages; bacteria-specific viruses) are readily isolated from environmental samples. However, it remains challenging to characterize phage-bacteria interactions, such as the host receptor(s) phages bind to initiate infection. Here, we tested whether transposon insertion sequencing (INSeq) could be used to identify bacterial genes involved in phage binding. As proof of concept, results showed that INSeq screens successfully identified genes encoding known receptors for previously characterized viruses of Escherichia coli (phages T6, T2, T4, and T7). INSeq screens were then used to identify genes involved during infection of six newly isolated coliphages. Results showed that candidate receptors could be successfully identifi

SUBMITTER: Kortright KE 

PROVIDER: S-EPMC7414163 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature