Heterotrimeric G Stimulatory Protein α Subunit Is Required for Intestinal Smooth Muscle Contraction in Mice.

ABSTRACT:

Background & aims

The α subunit of the heterotrimeric G stimulatory protein (Gsa), encoded by the guanine nucleotide binding protein, α-stimulating gene (Gnas, in mice), is expressed ubiquitously and mediates receptor-stimulated production of cyclic adenosine monophosphate and activation of the protein kinase A signaling pathway. We investigated the roles of Gsa in vivo in smooth muscle cells of mice.

Methods

We performed studies of mice with Cre recombinase-mediated disruption of Gnas in smooth muscle cells (Gsa^SMKO and SM22-CreER^T2, induced in adult mice by tamoxifen). Intestinal tissues were collected for histologic, biochemical, molecular, cell biology, and physiology analyses. Intestinal function was assessed in mice using the whole-gut transit tim

SUBMITTER: Qin X 

PROVIDER: S-EPMC7430528 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature