Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents.

ABSTRACT:

Background

Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6-8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. Moreover, the application of conventional slow-freezing methods to cultures of 3-D organoids of stem cells in various studies, is limited by their size.

Results

In this study, we evaluated the effect of high concentrations of CPAs including cytotoxicity and characterized human mesenchymal stem cell (MSC) at single cell level. The cell viability, cellular damage, and apoptotic mechanisms as well as the proliferation capacity and multipotency of cells subjected to vitrification were similar to those in the

SUBMITTER: Jeong YH 

PROVIDER: S-EPMC7449025 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature