Improving Proteoform Identifications in Complex Systems Through Integration of Bottom-Up and Top-Down Data.

ABSTRACT: Cellular functions are performed by a vast and diverse set of proteoforms. Proteoforms are the specific forms of proteins produced as a result of genetic variations, RNA splicing, and post-translational modifications (PTMs). Top-down mass spectrometric analysis of intact proteins enables proteoform identification, including proteoforms derived from sequence cleavage events or harboring multiple PTMs. In contrast, bottom-up proteomics identifies peptides, which necessitates protein inference and does not yield proteoform identifications. We seek here to exploit the synergies between these two data types to improve the quality and depth of the overall proteomic analysis. To this end, we automated the large-scale integration of results from multiprotease bottom-up and top-down analyses in the

SUBMITTER: Schaffer LV 

PROVIDER: S-EPMC7490796 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature