Fast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles.

ABSTRACT: Despite broad application of magnetic nanoparticles in biomedicine and electronics, only a few in vivo studies on biocompatibility are available. In this study, toxicity of magnetic metal oxide nanoparticles on the respiratory system was examined in vivo by single intratracheal instillation in mice. Bronchoalveolar lavage fluid (BALF) samples were collected for proteome analyses by LC-MS/MS, testing Fe₃O₄ nanoparticles doped with increasing amounts of cobalt (Fe₃O₄, CoFe₂O₄ with an iron to cobalt ratio 5:1, 3:1, 1:3, Co₃O₄) at two doses (54 μg, 162 μg per animal) and two time points (day 1 and 3 days postinstillation). In discovery phase, in-depth proteome profiling of a few representative

SUBMITTER: Billing AM 

PROVIDER: S-EPMC7498156 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature