ALDH-1-positive cells exhibited a radioresistant phenotype that was enhanced with hypoxia in cervical cancer.

ABSTRACT:

Background

We have previously found there was a small subpopulation of cells with cancer stem cell-like phenotype ALDH-1 in cervical cancer. Radiotherapy has been applied in most of the cervical cancer. However,the mechanisms underlying radioresistance still remained elusive. Our study is to explore whether ALDH+ cell promotes radioresistance by hypoxia.

Methods

Cells were respectively cultured in hypoxia and normoxia environment and analyzed for marker stability, and cell cycle distribution.

Results

Cell growth, apoptosis, cell cycle, sphere formation were affected by hypoxia. ALDH-1 and CHK2 were upregulated after hypoxia.

Conclusions

Here we show that ALDH-1 positive cells contribute to cervical carcinoma radioresistance through preferential activation of th

SUBMITTER: Yao T 

PROVIDER: S-EPMC7499852 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature