Dataset Information

MicroRNA-17-5p regulates EMT by targeting vimentin in colorectal cancer.

ABSTRACT:

Background

Epithelial-mesenchymal transition (EMT) is the most common cause of death in colorectal cancer (CRC). In this study, we investigated the functional roles of miRNA-17-5p in EMT of CRC cells.

Methods

In order to determine if miRNA-17-5p regulated EMT, the precursors and inhibitors of miR-17-5p were transduced into four CRC cells. To evaluate the regulatory mechanism, we performed argonaute 2 (Ago2) immunoprecipitation (IP) and luciferase assay. In addition, we used an intra-splenic injection mouse model of BALB/c nude mice to investigate the metastatic potential of miRNA-17-5p in vivo.

Results

The miRNA-17-5p expression was lower in primary CRC tissues with metastasis than in primary CRC tissues without metastasis in our RNA sequencing data of patient tissue. Real-time quantitative PCR revealed that miRNA-17-5p was inversely correlated with that of vimentin in five CRC cell lines. Over-expression of miRNA-17-5p decreased vimentin expression and inhibited cell migration and invasion in both LoVo and HT29 cells. However, inhibition of miRNA-17-5p showed the opposite effect. Ago2 IP and luciferase assay revealed that miRNA-17-5p directly bound to the 3'UTR of VIM mRNA. Furthermore, miRNA-17-5p inhibited the metastasis of CRC into liver in vivo.

Conclusions

Our results demonstrated that miRNA-17-5p regulates vimentin expression, thereby regulating metastasis of CRC.

SUBMITTER: Kim TW

PROVIDER: S-EPMC7524803 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Publications

MicroRNA-17-5p regulates EMT by targeting vimentin in colorectal cancer.

Kim Tae Won TW Lee Yeo Song YS Yun Nak Hyeon NH Shin Chang Hoon CH Hong Hye Kyung HK Kim Hyeon Ho HH Cho Yong Beom YB

British journal of cancer 20200617 7

<h4>Background</h4>Epithelial-mesenchymal transition (EMT) is the most common cause of death in colorectal cancer (CRC). In this study, we investigated the functional roles of miRNA-17-5p in EMT of CRC cells.<h4>Methods</h4>In order to determine if miRNA-17-5p regulated EMT, the precursors and inhibitors of miR-17-5p were transduced into four CRC cells. To evaluate the regulatory mechanism, we performed argonaute 2 (Ago2) immunoprecipitation (IP) and luciferase assay. In addition, we used an int ...[more]

PMID: 32546833

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Dataset Information

MicroRNA-17-5p regulates EMT by targeting vimentin in colorectal cancer.

Background

Methods

Results

Conclusions

Publications

MicroRNA-17-5p regulates EMT by targeting vimentin in colorectal cancer.

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