Lymphocyte Activation Gene (LAG)-3 Is Associated With Mucosal Inflammation and Disease Activity in Ulcerative Colitis.

ABSTRACT:

Background and aims

Lymphocyte activation gene [LAG]-3 is an immune checkpoint and its expression identifies recently activated lymphocytes that may contribute to inflammation. We investigated the role of LAG-3 by analysing its expression and function in immune cells from blood and tissue of patients with ulcerative colitis [UC].

Methods

The phenotypic properties of LAG-3+ T cells were determined by flow cytometry, qRT-PCR and single-cell RNA-sequencing. LAG-3+ cells were quantified and correlated with disease activity. The functional effects of LAG-3+ cells were tested using a depleting anti-LAG-3 monoclonal antibody [mAb] in a mixed lymphocyte reaction [MLR].

Results

LAG-3+ cells in the blood were negligible. LAG-3+ lymphocytes were markedly increased in inflamed mu

SUBMITTER: Slevin SM 

PROVIDER: S-EPMC7533903 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature