Role of METTL3-Dependent N⁶-Methyladenosine mRNA Modification in the Promotion of Angiogenesis.

ABSTRACT: Epigenetic alterations occur in many physiological and pathological processes. N⁶-methyladenosine (m⁶A) modification is the most prevalent modification in eukaryotic mRNAs. However, the role of m⁶A modification in pathological angiogenesis remains elusive. In this study, we showed that the level of m⁶A modification was significantly upregulated in endothelial cells and mouse retinas following hypoxic stress, which was caused by increased METTL3 levels. METTL3 silencing or METTL3 overexpression altered endothelial cell viability, proliferation, migration, and tube formation in vitro. METTL3 knockout in vivo decreased avascular area and pathological neovascular tufts in an oxygen-induced retinopathy model and inhibited alkali burn-induced corneal n

SUBMITTER: Yao MD 

PROVIDER: S-EPMC7545007 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature