Imaging Cardiovascular and Lung Macrophages With the Positron Emission Tomography Sensor ⁶⁴Cu-Macrin in Mice, Rabbits, and Pigs.

ABSTRACT:

Background

Macrophages, innate immune cells that reside in all organs, defend the host against infection and injury. In the heart and vasculature, inflammatory macrophages also enhance tissue damage and propel cardiovascular diseases.

Methods

We here use in vivo positron emission tomography (PET) imaging, flow cytometry, and confocal microscopy to evaluate quantitative noninvasive assessment of cardiac, arterial, and pulmonary macrophages using the nanotracer ⁶⁴Cu-Macrin-a 20-nm spherical dextran nanoparticle assembled from nontoxic polyglucose.

Results

PET imaging using ⁶⁴Cu-Macrin faithfully reported accumulation of macrophages in the heart and lung of mice with myocardial infarction, sepsis, or pneumonia. Flow cytometry and confocal microscopy

SUBMITTER: Nahrendorf M 

PROVIDER: S-EPMC7583675 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature