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An In Vitro Model for Identifying Cardiac Side Effects of Anesthetics.

ABSTRACT: The understanding of anesthetic side effects on the heart has been hindered by the lack of sophisticated clinical models. Using micropatterned human-induced pluripotent stem cell-derived cardiomyocytes, we obtained cardiac muscle depressant profiles for propofol, etomidate, and our newly identified anesthetic compound KSEB01-S2. Propofol was the strongest depressant among the 3 compounds tested, exhibiting the largest decrease in contraction velocity, depression rate, and beating frequency. Interestingly, KSEB01-S2 behaved similarly to etomidate, suggesting a better cardiac safety profile. Our results provide a proof-of-concept for using human-induced pluripotent stem cell-derived cardiomyocytes as an in vitro platform for future drug design.

SUBMITTER: Chang ACY

PROVIDER: S-EPMC7584389 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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An In Vitro Model for Identifying Cardiac Side Effects of Anesthetics.

Chang Alex C Y ACY Chang Andrew C H ACH Nicin Luka L Weber Gerhard J GJ Holbrook Colin C Davies M Frances MF Blau Helen M HM Bertaccini Edward J EJ

Anesthesia and analgesia 20200101 1

The understanding of anesthetic side effects on the heart has been hindered by the lack of sophisticated clinical models. Using micropatterned human-induced pluripotent stem cell-derived cardiomyocytes, we obtained cardiac muscle depressant profiles for propofol, etomidate, and our newly identified anesthetic compound KSEB01-S2. Propofol was the strongest depressant among the 3 compounds tested, exhibiting the largest decrease in contraction velocity, depression rate, and beating frequency. Inte ...[more]

PMID: 30198930

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