Hepatic miR-192-3p reactivation alleviates steatosis by targeting glucocorticoid receptor.

ABSTRACT:

Background & aims

The paradox of hepatic insulin resistance describes the inability for liver to respond to bioenergetics hormones in suppressing gluconeogenesis whilst maintaining lipid synthesis. Here, we report the deficiency of miR-192-3p in the livers of mice with diabetes and its role in alleviating hepatic steatosis.

Methods

As conventional pre-microRNA (miRNA) stem-loop overexpression only boosts guiding strand (i.e. miR-192-5p) expression, we adopted an artificial AAV(DJ)-directed, RNA Pol III promoter-driven miRNA hairpin construct for star-strand-specific overexpression in the liver. Liver steatosis and insulin resistance markers were evaluated in primary hepatocytes, mice with diabetes, and mice with excessive carbohydrate consumption.

Results

Funct

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7588854 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature