Downregulation of miR-192 Alleviates Oxidative Stress-Induced Porcine Granulosa Cell Injury by Directly Targeting Acvr2a.

ABSTRACT: Follicular atresia is primarily caused by breakdown to granulosa cells (GCs) due to oxidative stress (OS). MicroRNAs (miRNAs) elicit a defense response against environmental stresses, such as OS, by acting as gene-expression regulators. However, the association between miRNA expression and OS in porcine GCs (PGCs) is unclear. Here, we examined the impact of H₂O₂-mediated OS in PGCs through miRNA-Seq. We identified 22 (14 upregulated and 8 downregulated) and 33 (19 upregulated and 14 downregulated) differentially expressed miRNAs (DEmiRNAs) at 100 μM and 300 μM H₂O₂, respectively, compared with the control group. Among the DEmiRNAs, mi-192 was most induced by H₂O₂-mediated OS, and the downregulation of miR-192 alleviated PGC

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9368079 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature