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Event-driven acquisition for content-enriched microscopy.

ABSTRACT: A common goal of fluorescence microscopy is to collect data on specific biological events. Yet, the event-specific content that can be collected from a sample is limited, especially for rare or stochastic processes. This is due in part to photobleaching and phototoxicity, which constrain imaging speed and duration. We developed an event-driven acquisition framework, in which neural-network-based recognition of specific biological events triggers real-time control in an instant structured illumination microscope. Our setup adapts acquisitions on-the-fly by switching between a slow imaging rate while detecting the onset of events, and a fast imaging rate during their progression. Thus, we capture mitochondrial and bacterial divisions at imaging rates that match their dynamic timescales, while extending overall imaging durations. Because event-driven acquisition allows the microscope to respond specifically to complex biological events, it acquires data enriched in relevant content.

SUBMITTER: Mahecic D

PROVIDER: S-EPMC7613693 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Event-driven acquisition for content-enriched microscopy.

Mahecic Dora D Stepp Willi L WL Zhang Chen C Griffié Juliette J Weigert Martin M Manley Suliana S

Nature methods 20220908 10

A common goal of fluorescence microscopy is to collect data on specific biological events. Yet, the event-specific content that can be collected from a sample is limited, especially for rare or stochastic processes. This is due in part to photobleaching and phototoxicity, which constrain imaging speed and duration. We developed an event-driven acquisition framework, in which neural-network-based recognition of specific biological events triggers real-time control in an instant structured illumin ...[more]

PMID: 36076039

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