Acoustophoretic Characterization and Separation of Blood Cells in Acoustic Impedance Gradients.

ABSTRACT: Single-cell phenotyping based on biophysical properties is a promising tool to distinguish cell types and their response to a given condition, and charting such properties also enables optimization of cell separations. Isoacoustic focusing, where cells migrate to their points of zero acoustic contrast in an acoustic impedance gradient, added the effective acoustic impedance of cells to the directory of biophysical properties that can be utilized to categorize or separate cells. This study investigates isoacoustic focusing in a stop-flow regime and shows how cells migrate towards their isoacoustic point. We introduce a numerical model that we use to estimate the acoustic energy density in acoustic impedance gradient media by tracking particles of known properties, and we investigate the eff

SUBMITTER: Charan MR 

PROVIDER: S-EPMC7615610 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature