Evidence of Calcium Signaling and Modulation of the LmrS Multidrug Resistant Efflux Pump Activity by Ca^{2 +} Ions in S. aureus.

ABSTRACT: Calcium ions (Ca²⁺) play a pivotal role in eukaryote cell signaling and regulate many physiological functions. Although a similar role for Ca²⁺ in prokaryotes has been difficult to demonstrate, there is increasing evidence for Ca²⁺ as a cell regulator in bacteria. The purpose of this study was to investigate Ca²⁺ signaling and the effect of Ca²⁺ on the Staphylococcus aureus multidrug resistant efflux pump LmrS. We hypothesized that antibiotics act by increasing Ca²⁺ concentrations, which in turn enhance the efflux activity of LmrS. These Ca²⁺ transients were measured by luminometry in response to various antibiotics by using the photoprotein aequorin reconstituted within live bacterial cells. Efflux associa

SUBMITTER: Nava AR 

PROVIDER: S-EPMC7642317 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature