Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol.

ABSTRACT:

Background

Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to address complex biological problems such as toxicity.

Results

Here we applied this method successfully to evolve E. coli towards higher tolerance against isoprenol, increasing growth at the half-maximal inhibitory concentration by 47%. Whole-genome re-sequencing of strains isolated from three replicate evolutions at seven time-points identified four major target genes for isoprenol tolerance: fabF, marC, yghB, and rob. We could show that knock-out of marC and expression of mutated Rob H(48) → 

SUBMITTER: Babel H 

PROVIDER: S-EPMC7653855 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature