A T-cell reporter platform for high-throughput and reliable investigation of TCR function and biology.

ABSTRACT:

Objective

Transgenic re-expression enables unbiased investigation of T-cell receptor (TCR)-intrinsic characteristics detached from its original cellular context. Recent advancements in TCR repertoire sequencing and development of protocols for direct cloning of full TCRαβ constructs now facilitate large-scale transgenic TCR re-expression. Together, this offers unprecedented opportunities for the screening of TCRs for basic research as well as clinical use. However, the functional characterisation of re-expressed TCRs is still a complicated and laborious matter. Here, we propose a Jurkat-based triple parameter TCR signalling reporter endogenous TCR knockout cellular platform (TPR^KO) that offers an unbiased, easy read-out of TCR functionality and facilitates high-throughpu

SUBMITTER: Muller TR 

PROVIDER: S-EPMC7681835 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature