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Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae.

ABSTRACT: A multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in Cornu Saigae Tataricae. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes. When a mixture of antelope hornand fake species was assayed, it exhibited powerful differentiation capability. 5 out of 12 batches of commercialproducts were identified to be counterfeited or adulterated with Ovis aries Linnaeus and/or Capra hircus Linnaeus. It is readily applicable in routine analysis for identification of sham or adulterants of Cornu Saigae Tataricae.

SUBMITTER: Chen Y

PROVIDER: S-EPMC7707849 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in <i>Cornu Saigae Tataricae</i>.

Chen Yufei Y Yang Yaya Y Qian Yinhuan Y Gblinwon Roselyn Tehzee RT Jiao Zhaoqun Z Chen Liqun L Lin Ling L Zheng Yang Y Yang Huan H Shen Yuping Y

Mitochondrial DNA. Part B, Resources 20190925 2

A multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in <i>Cornu Saigae Tataricae</i>. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes. When a mixture of antelope hornand fake species was assayed, it exhibited powerful differentiation capability. 5 out of 12 batches of commercialproducts were identified to be counterfeited or ...[more]

PMID: 33365929

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