Identifying Key MicroRNAs Targeted by Narenmandula in a Rodent Nephropathy Model.

ABSTRACT:

Background

Untreated nephropathy can progress to renal failure. The traditional Mongolian remedy Narenmandula regulates the kidney "yang." This study aimed to identify key microRNAs (miRNAs) targeted by Narenmandula in a rat model of nephropathy.

Methods

Fifteen rats exhibiting normal renal function were randomized to three study arms. Nephropathy was induced in n = 10 rats using doxorubicin hydrochloride, followed by either Narenmandula treatment (treatment group) or no treatment (control group). In n = 5 rats, no doxorubicin was given and renal function remained unchanged (healthy group). Microarray analysis identified miRNAs which were differentially expressed (DE-miRNAs) between groups. Target genes of DE-miRNAs were predicted using miRWalk version 2.0, fol

SUBMITTER: Wang X 

PROVIDER: S-EPMC7707998 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature