The ribosomal RNA m⁵C methyltransferase NSUN-1 modulates healthspan and oogenesis in <i>Caenorhabditis elegans</i>.

ABSTRACT: Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m⁵C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second known 26S rRNA m⁵C at position C2982. Depletion of nsun-1 or nsun-5 improved thermotolerance and slightly increased locomotion at midlife, however, only soma-specific knockdown of nsun-1 extended lifespan. Moreover, soma-specific knockdown of nsun-1 reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by nsun-1 deple

SUBMITTER: Heissenberger C 

PROVIDER: S-EPMC7746234 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature