PKN2 is involved in aggregation and spheroid formation of fibroblasts in suspension culture by regulating cell motility and N-cadherin expression.

ABSTRACT: The role of Protein Kinase N2 (PKN2, also known as PRK2/PKNγ) in cell aggregate/spheroid formation in suspension culture was investigated using immortalized fibroblasts established from PKN2 ^flox/flox mouse embryos. PKN2 ^flox/flox cells formed cell aggregates in flat bottom low attachment well plates, such as 2% agar and poly-2-hydroxyethymethacrylate coated plates, however, Cre;PKN2 ^flox/flox cells in which PKN2 was depleted by the introduction of Cre-recombinase rarely formed aggregates. Time-lapse analysis revealed that the velocity of Cre;PKN2 ^flox/flox cell motility was significantly lower than that of PKN2 ^flox/flox in a low attachment flat-bottom plate, which likely resulted in a lower cell-cel

SUBMITTER: Kubouchi K 

PROVIDER: S-EPMC7787963 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature