Engineered Plant-Based Nanocellulose Hydrogel for Small Intestinal Organoid Growth.

ABSTRACT: Organoids are three-dimensional self-renewing and organizing clusters of cells that recapitulate the behavior and functionality of developed organs. Referred to as "organs in a dish," organoids are invaluable biological models for disease modeling or drug screening. Currently, organoid culture commonly relies on an expensive and undefined tumor-derived reconstituted basal membrane which hinders its application in high-throughput screening, regenerative medicine, and diagnostics. Here, we introduce a novel engineered plant-based nanocellulose hydrogel is introduced as a well-defined and low-cost matrix that supports organoid growth. Gels containing 0.1% nanocellulose fibers (99.9% water) are ionically crosslinked and present mechanical properties similar to the standard animal-based matrix.

SUBMITTER: Curvello R 

PROVIDER: S-EPMC7788499 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature