Unraveling the roles of the reductant and free copper ions in LPMO kinetics.

ABSTRACT:

Background

Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative depolymerization of industrially relevant crystalline polysaccharides, such as cellulose, in a reaction that depends on an electron donor and O₂ or H₂O₂. While it is well known that LPMOs can utilize a wide variety of electron donors, the variation in reported efficiencies of various LPMO-reductant combinations remains largely unexplained.

Results

In this study, we describe a novel two-domain cellulose-active family AA10 LPMO from a marine actinomycete, which we have used to look more closely at the effects of the reductant and copper ions on the LPMO reaction. Our results show that ascorbate-driven LPMO reactions are extremely sensitive to v

SUBMITTER: Stepnov AA 

PROVIDER: S-EPMC7818938 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature