ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli.

ABSTRACT:

Objectives

The emergence of mobile colistin resistance genes has compromised the efficacy of the last resort antibiotic, colistin, in clinical treatment. The mcr-2 gene was first identified in Belgium in association with the insertion sequence ISEc69. However, the molecular mechanisms of mcr-2 mobilization are not well understood.

Methods

To further explore the mobilization of mcr-2 gene via ISEc69, we constructed a conjugative plasmid that carries an intact composite transposon Tn7052. Transposition assays were performed by conjugation, the transposition sites were characterized by arbitrary primed PCR and DNA sequencing.

Results

In this study, we experimentally demonstrated that mcr-2 could be mobilized as a composite transp

SUBMITTER: He YZ 

PROVIDER: S-EPMC7835479 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature