Distribution of β-Lactamase Genes and Genetic Context of bla _KPC-2 in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates.

ABSTRACT:

Background

This study was designed to characterize the dissemination mechanism and genetic context of Klebsiella pneumoniae carbapenemase (KPC) genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates.

Methods

A retrospective analysis was performed on CRKP strains isolated from a teaching hospital of Wenzhou Medical University during 2015-2017. Polymerase chain reaction (PCR)-based amplification and whole-genome sequencing (WGS) were used to analyze the genetic context of the bla _KPC-2 gene. Conjugation experiments were performed to evaluate the transferability of bla _KPC-2-bearing plasmids. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed to investigate the clonal relate

SUBMITTER: Liu H 

PROVIDER: S-EPMC7847768 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature