Maternal diabetes alters microRNA expression in fetal exosomes, human umbilical vein endothelial cells and placenta.

ABSTRACT:

Background

Exposure to diabetes in utero influences future metabolic health of the offspring. MicroRNAs (miRNA) are small noncoding RNAs that may contribute mechanistically to the effects on offspring imparted by diabetes mellitus (DM) during pregnancy. We hypothesized that exposure to DM during pregnancy influences select miRNAs in fetal circulation, in human umbilical vein endothelial cells (HUVEC), and placenta.

Methods

miRNA abundance was quantified using real-time PCR from RNA isolated from umbilical cord serum exosomes, HUVEC, and placenta exposed to diabetes or normoglycemia during pregnancy. The abundance of each of these miRNAs was determined by comparison to a known standard and the relative expression assessed using the 2^-ΔΔCt method. Multivariable regr

SUBMITTER: Shah KB 

PROVIDER: S-EPMC7854929 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature