Specific and Rapid SARS-CoV-2 Identification Based on LC-MS/MS Analysis.

ABSTRACT: SARS-CoV-2, the etiologic agent of the COVID-19 pandemic, emerged as the cause of a global crisis. Rapid and reliable clinical diagnosis is essential for effectively controlling transmission. The gold standard assay for SARS-CoV-2 identification is the highly sensitive real-time quantitative polymerase chain reaction (RT-qPCR); however, this assay depends on specialized reagents and may suffer from false results. Thus, additional assays based on different approaches could be beneficial. Here, we present a novel method for SARS-CoV-2 identification based on mass spectrometry. The approach we implemented combines a multistep procedure for the rational down-selection of a set of reliable markers out of all optional in silico derived tryptic peptides in viral proteins, followed by monit

SUBMITTER: Schuster O 

PROVIDER: S-EPMC7857140 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature