Ontology highlight
ABSTRACT:
SUBMITTER: Matsumura T
PROVIDER: S-EPMC7875995 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Matsumura Takafumi T Sato Takuya T Abe Takeru T Sanjo Hiroyuki H Katagiri Kumiko K Kimura Hiroshi H Fujii Teruo T Tanaka Hiromitsu H Hirabayashi Masumi M Ogawa Takehiko T
Scientific reports 20210210 1
In vitro spermatogenesis (IVS) using air-liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progression of spermatogenesis. In the present study, we challenged IVS of rats with modifications of culture medium, by supplementing chemical substances, including hormones, antioxidants, and lysophospholipids. In addition, reducing oxygen tension by placing tissues in ...[more]